Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers.
Identifieur interne : 000574 ( Main/Exploration ); précédent : 000573; suivant : 000575Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers.
Auteurs : R F Peixoto-Junior [Brésil] ; S. Creste [Brésil] ; M G A. Landell [Brésil] ; D S Nunes [Brésil] ; A. Sanguino [Brésil] ; M F Campos [Brésil] ; R. Vencovsky [Brésil] ; E V Tambarussi [Brésil] ; A. Figueira [Brésil]Source :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- Wicri :
- geographic : Brésil.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA Primers, Genetic Markers.
- geographic : Brazil.
- classification : Basidiomycota.
- genetics : Basidiomycota.
- Base Sequence, Genetic Variation, Microsatellite Repeats.
Abstract
Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the genetic diversity of 34 P. melanocephala isolates from 4 Brazilian states using loci identified from an enriched simple sequence repeat (SSR) library. The aggressiveness of 3 isolates from major sugarcane cultivation areas was evaluated by inoculating an intermediately resistant and a susceptible cultivar. From the enriched library, 16 SSR-specific primers were developed, which produced scorable alleles. Of these, 4 loci were polymorphic and 12 were monomorphic for all isolates evaluated. The molecular characterization of the 34 isolates of P. melanocephala conducted using 16 SSR loci revealed the existence of low genetic variability among the isolates. The average estimated genetic distance was 0.12. Phenetic analysis based on Nei's genetic distance clustered the isolates into 2 major groups. Groups I and II included 18 and 14 isolates, respectively, and both groups contained isolates from all 4 geographic regions studied. Two isolates did not cluster with these groups. It was not possible to obtain clusters according to location or state of origin. Analysis of disease severity data revealed that the isolates did not show significant differences in aggressiveness between regions.
DOI: 10.4238/2014.September.26.23
PubMed: 25299099
Affiliations:
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Creste</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil screste@iac.sp.gov.br.</nlm:affiliation><country wicri:rule="url">Brésil</country><wicri:regionArea>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Landell, M G A" sort="Landell, M G A" uniqKey="Landell M" first="M G A" last="Landell">M G A. Landell</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Nunes, D S" sort="Nunes, D S" uniqKey="Nunes D" first="D S" last="Nunes">D S Nunes</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Sanguino, A" sort="Sanguino, A" uniqKey="Sanguino A" first="A" last="Sanguino">A. Sanguino</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Campos, M F" sort="Campos, M F" uniqKey="Campos M" first="M F" last="Campos">M F Campos</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Vencovsky, R" sort="Vencovsky, R" uniqKey="Vencovsky R" first="R" last="Vencovsky">R. Vencovsky</name><affiliation wicri:level="4"><nlm:affiliation>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Tambarussi, E V" sort="Tambarussi, E V" uniqKey="Tambarussi E" first="E V" last="Tambarussi">E V Tambarussi</name><affiliation wicri:level="4"><nlm:affiliation>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Figueira, A" sort="Figueira, A" uniqKey="Figueira A" first="A" last="Figueira">A. Figueira</name><affiliation wicri:level="4"><nlm:affiliation>Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brasil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Basidiomycota (classification)</term><term>Basidiomycota (genetics)</term><term>Brazil (MeSH)</term><term>DNA Primers (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Microsatellite Repeats (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Basidiomycota (classification)</term><term>Basidiomycota (génétique)</term><term>Brésil (MeSH)</term><term>Marqueurs génétiques (MeSH)</term><term>Répétitions microsatellites (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Genetic Markers</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Brazil</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genetic Variation</term><term>Microsatellite Repeats</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Basidiomycota</term><term>Brésil</term><term>Marqueurs génétiques</term><term>Répétitions microsatellites</term><term>Séquence nucléotidique</term><term>Variation génétique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Brésil</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the genetic diversity of 34 P. melanocephala isolates from 4 Brazilian states using loci identified from an enriched simple sequence repeat (SSR) library. The aggressiveness of 3 isolates from major sugarcane cultivation areas was evaluated by inoculating an intermediately resistant and a susceptible cultivar. From the enriched library, 16 SSR-specific primers were developed, which produced scorable alleles. Of these, 4 loci were polymorphic and 12 were monomorphic for all isolates evaluated. The molecular characterization of the 34 isolates of P. melanocephala conducted using 16 SSR loci revealed the existence of low genetic variability among the isolates. The average estimated genetic distance was 0.12. Phenetic analysis based on Nei's genetic distance clustered the isolates into 2 major groups. Groups I and II included 18 and 14 isolates, respectively, and both groups contained isolates from all 4 geographic regions studied. Two isolates did not cluster with these groups. It was not possible to obtain clusters according to location or state of origin. Analysis of disease severity data revealed that the isolates did not show significant differences in aggressiveness between regions. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25299099</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>25</Day></DateCompleted><DateRevised><Year>2014</Year><Month>10</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1676-5680</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Sep</Month><Day>26</Day></PubDate></JournalIssue><Title>Genetics and molecular research : GMR</Title><ISOAbbreviation>Genet Mol Res</ISOAbbreviation></Journal><ArticleTitle>Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers.</ArticleTitle><Pagination><MedlinePgn>7852-63</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4238/2014.September.26.23</ELocationID><Abstract><AbstractText>Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the genetic diversity of 34 P. melanocephala isolates from 4 Brazilian states using loci identified from an enriched simple sequence repeat (SSR) library. The aggressiveness of 3 isolates from major sugarcane cultivation areas was evaluated by inoculating an intermediately resistant and a susceptible cultivar. From the enriched library, 16 SSR-specific primers were developed, which produced scorable alleles. Of these, 4 loci were polymorphic and 12 were monomorphic for all isolates evaluated. The molecular characterization of the 34 isolates of P. melanocephala conducted using 16 SSR loci revealed the existence of low genetic variability among the isolates. The average estimated genetic distance was 0.12. Phenetic analysis based on Nei's genetic distance clustered the isolates into 2 major groups. Groups I and II included 18 and 14 isolates, respectively, and both groups contained isolates from all 4 geographic regions studied. Two isolates did not cluster with these groups. It was not possible to obtain clusters according to location or state of origin. Analysis of disease severity data revealed that the isolates did not show significant differences in aggressiveness between regions. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Peixoto-Junior</LastName><ForeName>R F</ForeName><Initials>RF</Initials><AffiliationInfo><Affiliation>Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Creste</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil screste@iac.sp.gov.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Landell</LastName><ForeName>M G A</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nunes</LastName><ForeName>D S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sanguino</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Campos</LastName><ForeName>M F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Centro de Cana, Instituto Agronômico de Campinas, Ribeirão Preto, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vencovsky</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tambarussi</LastName><ForeName>E V</ForeName><Initials>EV</Initials><AffiliationInfo><Affiliation>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Figueira</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brasil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Genet Mol Res</MedlineTA><NlmUniqueID>101169387</NlmUniqueID><ISSNLinking>1676-5680</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001938" MajorTopicYN="N" Type="Geographic">Brazil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018895" MajorTopicYN="Y">Microsatellite Repeats</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>10</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>10</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>6</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25299099</ArticleId><ArticleId IdType="pii">gmr4498</ArticleId><ArticleId IdType="doi">10.4238/2014.September.26.23</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Peixoto Junior, R F" sort="Peixoto Junior, R F" uniqKey="Peixoto Junior R" first="R F" last="Peixoto-Junior">R F Peixoto-Junior</name></region><name sortKey="Campos, M F" sort="Campos, M F" uniqKey="Campos M" first="M F" last="Campos">M F Campos</name><name sortKey="Creste, S" sort="Creste, S" uniqKey="Creste S" first="S" last="Creste">S. Creste</name><name sortKey="Figueira, A" sort="Figueira, A" uniqKey="Figueira A" first="A" last="Figueira">A. Figueira</name><name sortKey="Landell, M G A" sort="Landell, M G A" uniqKey="Landell M" first="M G A" last="Landell">M G A. Landell</name><name sortKey="Nunes, D S" sort="Nunes, D S" uniqKey="Nunes D" first="D S" last="Nunes">D S Nunes</name><name sortKey="Sanguino, A" sort="Sanguino, A" uniqKey="Sanguino A" first="A" last="Sanguino">A. Sanguino</name><name sortKey="Tambarussi, E V" sort="Tambarussi, E V" uniqKey="Tambarussi E" first="E V" last="Tambarussi">E V Tambarussi</name><name sortKey="Vencovsky, R" sort="Vencovsky, R" uniqKey="Vencovsky R" first="R" last="Vencovsky">R. Vencovsky</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RustFungiGenomicsV1
| This area was generated with Dilib version V0.6.38. |  |